Tg(ACTA1-UCP3)1Clap
Transgene Detail

Summary

• Symbol: Tg(ACTA1-UCP3)1Clap
• Name: transgene insertion 1, John C Clapham
• MGI ID: MGI:2446449
• Synonyms: UCP-3tg
• Transgene: Tg(ACTA1-UCP3)1Clap Location: unknown
• Alliance: Tg(ACTA1-UCP3)1Clap page

Transgene origin

• Strain of Origin: (C57BL/6 x CBA)F2

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(ACTA1-UCP3)1Clap expresses 1 gene
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  UCP3 (7352)

  Ucp3	(MGI:1099787)

• Mutation details: The transgene consists of a human ACTA1 promoter and a human UCP3 gene. ACTA1 drives transgene expression in skeletal muscle. (J:69792)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

Notes

Three transgenic lines were created and bred to homozygosity. The highest expressing line was used for characterization. Quantitative RT-PCR analysis showed that transgene expression is confined to skeletal muscle and brown adipose tissue. Transgene expression is 66-fold of wild-type in skeletal muscle and 50% of wild-type in brown adipose tissue.

References

• Original: J:69792 Clapham JC, et al., Mice overexpressing human uncoupling protein-3 in skeletal muscle are hyperphagic and lean. Nature. 2000 Jul 27;406(6794):415-8
• All: 13 reference(s)