Slc6a3^tm2Mca
Targeted Allele Detail

Summary

• Symbol: Slc6a3^tm2Mca
• Name: solute carrier family 6 (neurotransmitter transporter, dopamine), member 3; targeted mutation 2, Marc G Caron
• MGI ID: MGI:2446518
• Synonyms: DAT-KD
• Gene: Slc6a3 Location: Chr13:73684866-73726791 bp, + strand Genetic Position: Chr13, 40.1 cM
• Alliance: Slc6a3^tm2Mca page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:67549
• Parent Cell Line: W9.5/W95 (ES Cell)
• Strain of Origin: 129S1/Sv-Oca2⁺ Tyr⁺ Kitl⁺

Mutation description

• Allele Type: Targeted (Hypomorph)
• Mutation: Insertion
• Mutation details: A hypomorphic allele was generated by the insertion of a cassette containing tTA, PGK-neo, and tetO immediately upstream of the start codon. While expression of tTA was not detected, putatively due to an influence of the PGK promoter, Western blot analysis of homozygous mutant mice showed that the presence of the cassette reduced protein levels by approximately 90% in the striatum. (J:67549)

Disease models

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Slc6a3 Mutation: 66 strains or lines available

References

• Original: J:67549 Zhuang X, et al., Hyperactivity and impaired response habituation in hyperdopaminergic mice. Proc Natl Acad Sci U S A. 2001 Feb 13;98(4):1982-7
• All: 18 reference(s)