Tg(NEFL-APP)#Nalb
Transgene Detail

Summary

• Symbol: Tg(NEFL-APP)#Nalb
• Name: transgene insertion, Josephine Nalbantoglu
• MGI ID: MGI:2446543
• Synonyms: APP(C104)
• Transgene: Tg(NEFL-APP)#Nalb Location: unknown
• Alliance: Tg(NEFL-APP)#Nalb page

Transgene origin

• Strain of Origin: (C57BL/6 x C3H)F1

Transgene description

• Transgene Type: Transgenic (Humanized sequence, Inserted expressed sequence)
• Mutation: Insertion
• Tg(NEFL-APP)#Nalb expresses 1 gene
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  APP (351)

  App	(MGI:88059)

• Mutation details: Human APP sequence encoding amino acids 591 through 695 was adjoined to the human NEFL regulatory sequences as well as the first exon. Northern blot analysis showed expression of the amyloid derived transcripts, approximately 900 nt long, at levels similar to the endogenous App in the brains of transgenic mice. Western blot analysis using an antibody raised against the beta-amyloid peptide showed a pattern of bands that correlates with carboxy terminal fragments identified in human brain. Three lines were established. The pound symbol (#) is used when line is not specified and/or lines are pooled. (J:80639)

Disease models

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:80639 Nalbantoglu J, et al., Impaired learning and LTP in mice expressing the carboxy terminus of the Alzheimer amyloid precursor protein. Nature. 1997 May 29;387(6632):500-5
• All: 2 reference(s)