Ltb/Tnftm1.1Dvk
Targeted Allele Detail
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Symbol: |
Ltb/Tnftm1.1Dvk |
Name: |
lymphotoxin B; targeted mutation 1.1, Dmitry V Kuprash |
MGI ID: |
MGI:2446564 |
Synonyms: |
(LTBeta/TNF)delta |
Gene: |
Ltb Location: Chr17:35413483-35415281 bp, + strand Genetic Position: Chr17, 18.59 cM
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Alliance: |
Ltb/Tnftm1.1Dvk page
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Germline Transmission: |
Earliest citation of germline transmission:
J:58981
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Parent Cell Line: |
E14.1 (ES Cell)
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Strain of Origin: |
129P2/OlaHsd
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Allele Type: |
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Targeted (Null/knockout) |
Mutation: |
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Intergenic deletion
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Mutation details: The floxed 5.3 kb region, containing exon 3 of the Ltb locus and exons 3 and 4 of the Tnf locus, was excised via in vivo cre mediated recombination between the loxP site within the Ltb locus and the loxP site within the Tnf locus. The deleted regions encoded the majority of the extracellular region of Ltb and the entire secreted portion of Tnf. An RNAse protection assay using homozygous mutant concavalin A stimulated splenocytes detected a truncated Ltb transcript and demonstrated normal transcription of the closely linked Lta locus.
(J:58981)
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View phenotypes and curated references for all genotypes (concatenated display).
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Original: |
J:58981 Kuprash DV, et al., TNF and lymphotoxin beta cooperate in the maintenance of secondary lymphoid tissue microarchitecture but not in the development of lymph nodes. J Immunol. 1999 Dec 15;163(12):6575-80 |
All: |
3 reference(s) |
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