Ltb/Tnf^tm1.1Dvk
Targeted Allele Detail

Summary

• Symbol: Ltb/Tnf^tm1.1Dvk
• Name: tumor necrosis factor; targeted mutation 1.1, Dmitry V Kuprash
• MGI ID: MGI:2446565
• Synonyms: (LTBeta/TNF)^delta
• Gene: Tnf Location: Chr17:35418357-35420983 bp, - strand Genetic Position: Chr17, 18.59 cM
• Alliance: Ltb/Tnf^tm1.1Dvk page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:58981
• Parent Cell Line: E14.1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intergenic deletion
• Mutation details: The floxed 5.3 kb region, containing exon 3 of the Ltb locus and exons 3 and 4 of the Tnf locus, was excised via in vivo cre mediated recombination between the loxP site within the Ltb locus and the loxP site within the Tnf locus. The deleted regions encoded the majority of the extracellular region of Ltb and the entire secreted portion of Tnf. An RNAse protection assay using homozygous mutant concavalin A stimulated splenocytes detected a truncated Ltb transcript and demonstrated normal transcription of the closely linked Lta locus. (J:58981)

Expression

In Structures Affected by this Mutation:

6 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Tnf Mutation: 48 strains or lines available

References

• Original: J:58981 Kuprash DV, et al., TNF and lymphotoxin beta cooperate in the maintenance of secondary lymphoid tissue microarchitecture but not in the development of lymph nodes. J Immunol. 1999 Dec 15;163(12):6575-80
• All: 3 reference(s)