Ltb/Tnf/Lta^tm1Dvk
Targeted Allele Detail

Summary

• Symbol: Ltb/Tnf/Lta^tm1Dvk
• Name: tumor necrosis factor; targeted mutation 1, Dmitry V Kuprash
• MGI ID: MGI:2446567
• Synonyms: Tnfabc^-, TNF/LTDelta3
• Gene: Tnf Location: Chr17:35418357-35420983 bp, - strand Genetic Position: Chr17, 18.59 cM
• Alliance: Ltb/Tnf/Lta^tm1Dvk page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:80616
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intergenic deletion
• Mutation details: An 8.4 kb region, containing the entire Tnf gene and the last coding exons of the Lta and Ltb genes, was deleted by cre mediated recombination of surrounding loxP sites inserted within Lta and Ltb via homologous recombination. Partial coding regions of Lta and Ltb were left intact separated by a single loxP site, however RT-PCR analysis of homozygous mutant splenocytes activated by concanavalin A indicated an absence of transcript from each locus. (J:80616)

Expression

In Structures Affected by this Mutation:

10 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Tnf Mutation: 48 strains or lines available

References

• Original: J:80616 Kuprash DV, et al., Redundancy in tumor necrosis factor (TNF) and lymphotoxin (LT) signaling in vivo: mice with inactivation of the entire TNF/LT locus versus single-knockout mice. Mol Cell Biol. 2002 Dec;22(24):8626-34
• All: 9 reference(s)