Nts^tm1Dob
Targeted Allele Detail

Summary

• Symbol: Nts^tm1Dob
• Name: neurotensin; targeted mutation 1, Paul R Dobner
• MGI ID: MGI:2446607
• Synonyms: NT^-
• Gene: Nts Location: Chr10:102317617-102326294 bp, - strand Genetic Position: Chr10, 53.56 cM, cytoband D1
• Alliance: Nts^tm1Dob page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:80590
• Parent Cell Line: J1 (ES Cell)
• Strain of Origin: 129S4/SvJae

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Sequence encoding the precursor protein signal sequence was replaced with a cassette containing beta-galactosidase and neomyicin resistance genes. Transcript was undetected by Northern blot analysis of intestinal and brain samples obtained from homozygous mutant mice. Similarly, radioimmunoassay of HPLC-fractioned brain extracts and immunohistochemical analysis of brain sections indicated an absence of normal protein. Expression of the beta-galactosidase gene was not detected by staining. (J:80590)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Nts Mutation: 18 strains or lines available

References

• Original: J:80590 Dobner PR, et al., Neurotensin-deficient mice show altered responses to antipsychotic drugs. Proc Natl Acad Sci U S A. 2001 Jul 3;98(14):8048-53
• All: 8 reference(s)