Rr96^tm2Litt
Targeted Allele Detail

Summary

• Symbol: Rr96^tm2Litt
• Name: regulatory region 96; targeted mutation 2, Dan R Littman
• MGI ID: MGI:2446611
• Synonyms: CD4^S4delta, Cd4sil^-, Cd4^tm2Litt
• Gene: Rr96 Location: Chr6:124862841-124863329 bp Genetic Position: Chr6, Syntenic
• Alliance: Rr96^tm2Litt page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:80576
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Modified regulatory region, Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: The core region of a Cd4 cis-regulatory element that silences expression of the endogenous locus during T cell development (S4) was deleted from intron 1 via homologous recombination of a targeting construct lacking nucleotides 167 through 257. A single loxP site was left upstream of the deleted region, however analysis of a control allele indicated that the loxP site did not affect normal expression. Flow cytometric analysis of peripheral blood lymphocytes obtained from mutant mice showed no silencing of Cd4 expression. (J:80576)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rr96 Mutation: 0 strains or lines available

Notes

Additional alleles engineered to lack nucleotides within the core silencer and others engineered to lack surrounding sequences showed variegated expression of Cd4.

References

• Original: J:80576 Taniuchi I, et al., Evidence for distinct CD4 silencer functions at different stages of thymocyte differentiation. Mol Cell. 2002 Nov;10(5):1083-96
• All: 10 reference(s)