St3gal4^tm1.1Jxm
Targeted Allele Detail

Summary

• Symbol: St3gal4^tm1.1Jxm
• Name: ST3 beta-galactoside alpha-2,3-sialyltransferase 4; targeted mutation 1.1, Jamey Marth
• MGI ID: MGI:2446673
• Synonyms: ST3Gal-IV^-, ST3Gal-IV^delta
• Gene: St3gal4 Location: Chr9:34957872-35028160 bp, - strand Genetic Position: Chr9, 19.36 cM, cytoband A5.3
• Alliance: St3gal4^tm1.1Jxm page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:80884
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: Exons 5 through 7 were initially flanked by an upstream floxed tk-neo cassette and a downstream single loxP site, then deleted via cre mediated recombination in ES cells. In addition to lacking sequence encoding the large sialyl motif necessary for nucleotide sugar binding and enzymatic activity, the allele passed into the germline also contained a frameshift mutation within exon 8. Transcript was undetected by Northern blot analysis of homozygous mutant mice. (J:80884)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any St3gal4 Mutation: 26 strains or lines available

References

• Original: J:80884 Ellies LG, et al., Sialyltransferase ST3Gal-IV operates as a dominant modifier of hemostasis by concealing asialoglycoprotein receptor ligands. Proc Natl Acad Sci U S A. 2002 Jul 23;99(15):10042-7
• All: 22 reference(s)