Nobq2^NZO/HlBom
QTL Variant Detail

Summary

• QTL variant: Nobq2^NZO/HlBom
• Name: New Zealand obese QTL 2; NZO/HlBom
• MGI ID: MGI:2446833
• QTL: Nobq2 Location: Chr19:47897880-47897988 bp Genetic Position: Chr19, cM position of peak correlated region/allele: 40.53 cM
• QTL Note: genome coordinates based on the marker associated with the peak LOD score

Variant origin

• Strain of Specimen: NZO/HlBom

Variant description

• Allele Type: QTL
• Mutation: Undefined
• This allele confers increased serum insulin levels compared to SJL/NBom. (J:66744)
• Inheritance: Not Specified

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Notes

Candidate Genes

J:99477

Authors used novel data mining tool ExQuest to identify novel candidate genes for existing diabesity QTLs. Next, candidate gene expression in the liver, adipose, and pancreas of diabesity-prone Tally Ho mice and diabesity-resistant C57BL/6J mice was assessed by quantitative PCR analysis. Several potential candidate genes, some with no previous association to diabesity QTLs, were identified. Since QTL intervals may be large and could contain hundreds or thousands of potential candidate genes, this method allows researchers to focus on those with strong potential as well as identify novel candidate genes.

Potential candidate genes for Iba4 (20 cM) on mouse Chromosome 19 as identified by ExQuest are Aldh1a1 (12 cM), Aldh1a7, Vldlr (20 cM), and Plce1. Vldlr exhibits 15.6-fold increased expression in the liver of Tally Ho compared to C57BL/6 animals. For QTL Afw8 (26 cM), potential candidate genes Pi4k2a, Cpn1, and Elovl3 (47 cM) were identified. For QTLs Nobq2 (47 cM) and Bglq13 (51 cM), potential candidate genes Ins1 (49 cM), Gpam (52 cM), and Acsl5 were identified. For QTLs Tanidd1 (50 cM) and T2dm2 (53 cM), potential candidate genes Gfra1, Pnlip (29 cM), Pnliprp1 (29 cM), and Pnliprp2 were identified. Pnliprp2 exhibits 21.8-fold decreased expression in the pancreas of Tally Ho animals in response to a 4% fat diet.

Mapping and Phenotype information for this QTL, its variants and associated markers

J:66744

In NZO mice susceptibility to obesity is due in part to leptin resistance conferred by a variant Lepr allele, however this same alleic form is found in non-obese NZW mice suggesting there may be NZO genes that interact with Lepr to cause obesity. In a genome wide scan NZO x (SJL X NZO) F1 mice were informative in mapping two QTLs to mouse Chromosomes 5 and 19: Nob1 mapped with D5Mit392 whereas Nob2 mapped with D19Mit91.

References

• Original: J:66744 Kluge R, et al., Quantitative trait loci for obesity and insulin resistance (Nob1, Nob2) and their interaction with the leptin receptor allele (LeprA720T/T1044I) in New Zealand obese mice. Diabetologia. 2000 Dec;43(12):1565-72
• All: 2 reference(s)