Cd247^tm2Tks
Targeted Allele Detail

Summary

• Symbol: Cd247^tm2Tks
• Name: CD247 antigen; targeted mutation 2, Takashi Saito
• MGI ID: MGI:2446976
• Synonyms: Cd247/Pou2f1^tm1Tks, Cd247^tm1Tks, Cd3z/Pou2f1^tm1Tks, etaT, Pou2f1^tm1Tks
• Gene: Cd247 Location: Chr1:165616250-165704846 bp, + strand Genetic Position: Chr1, 73.14 cM, cytoband G-H
• Alliance: Cd247^tm2Tks page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:17251
• Parent Cell Line: E14.1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intergenic deletion
• Cd247^tm2Tks involves 1 genes/genome features (Pou2f1) View all
• Mutation details: The replacement of a 1.4 kb genomic fragment with a neo cassette resulted in the disruption of both the Cd247 and Pou2f1 loci, which are located at the same chromosomal position on opposite strands. The deletion of a 3' portion of Cd247 exon 9 and its splice acceptor site disrupted sequence encoding the eta isoform while leaving the sequence encoding the zeta isoform intact. Neither eta transcript nor protein was detected in homozygous mutant mice while normal expression of zeta chain homodimers was confirmed by 2D SDS-PAGE analysis. The insertion of the neo cassette also replaced the 3' UTR of the Pou2f1 locus, resulting in reduced expression of normal transcript as well as in an induced expression of aberrant transcript. (J:17251)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cd247 Mutation: 11 strains or lines available

References

• Original: J:17251 Ohno H, et al., Targeted disruption of the CD3 eta locus causes high lethality in mice: modulation of Oct-1 transcription on the opposite strand. EMBO J. 1994 Mar 1;13(5):1157-65
• All: 1 reference(s)