Ptdss2^{Gt(KST314)Byg}
Gene trapped Allele Detail

Summary

• Symbol: Ptdss2^{Gt(KST314)Byg}
• Name: phosphatidylserine synthase 2; gene trap KST314, BayGenomics
• MGI ID: MGI:2447151
• Synonyms: Pss2^-, Ptdss2^{Gt(pGT1.8TM)314Mbrg}
• Gene: Ptdss2 Location: Chr7:140711181-140736071 bp, + strand Genetic Position: Chr7, 86.39 cM
• Alliance: Ptdss2^{Gt(KST314)Byg} page

Mutation origin

• Mutant Cell Line: KST314
• Germline Transmission: Earliest citation of germline transmission: J:80673
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Gene trapped (Null/knockout, Reporter)
• Mutation: Insertion of gene trap vector Vector: pGT1TMpfs Vector Type: secretory trap
• Mutation details: The gene was disrupted by insertion of a gene trap vector (pGT1TMpfs) into intron 2. The resulting transcript is a fusion of the first 72 amino acids of Ptdss2 to the CD4 transmembrane domain and beta-geo sequences of the gene trap vector. Absence of wild-type transcript in homozygous mutant animals was confirmed by Northern blot analysis of testis and brain RNA. (J:70273, J:80673)
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  KST314	CC144693 ; (MGI Seq Detail)	5' RACE	Chr7:140711289-140711430 bp (+)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  79 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Expression

In Mice Carrying this Mutation:	19 assay results
In Structures Affected by this Mutation:	3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ptdss2 Mutation: 74 strains or lines available

Notes

All BayGenomics gene trap mutations were generated in either CGR8 or E14TG2a (129P2/OlaHsd) parental ES cell lines, with the majority in subline E14TG2a.4. The specific ES cell line in which each mutation was made is not specified.

References

• Original: J:80673 Bergo MO, et al., Defining the importance of phosphatidylserine synthase 2 in mice. J Biol Chem. 2002 Dec 6;277(49):47701-8
• All: 5 reference(s)