Guca2a^tm1.1Mcoh
Targeted Allele Detail

Summary

• Symbol: Guca2a^tm1.1Mcoh
• Name: guanylate cyclase activator 2a (guanylin); targeted mutation 1.1, Mitchell B Cohen
• MGI ID: MGI:2447173
• Gene: Guca2a Location: Chr4:119494929-119496662 bp, + strand Genetic Position: Chr4, 55.47 cM
• Alliance: Guca2a^tm1.1Mcoh page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:80545
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: Excision of the floxed 4.5 kb fragment (-1886 to +2714, numbering based reference J:80886) encompassing exon 3 and portions of introns 2 and 3 was achieved via in vivo cre mediated recombination. Though the endogenous promoter was left intact, truncated transcript was undetected by Northern blot analysis. Western blot analysis of ileum and distal colon tissue further indicated a complete disruption of expression. (J:80545)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Guca2a Mutation: 3 strains or lines available

References

• Original: J:80545 Steinbrecher KA, et al., Targeted inactivation of the mouse guanylin gene results in altered dynamics of colonic epithelial proliferation. Am J Pathol. 2002 Dec;161(6):2169-78
• All: 3 reference(s)