Ighm^tm1Msn
Targeted Allele Detail

Summary

• Symbol: Ighm^tm1Msn
• Name: immunoglobulin heavy constant mu; targeted mutation 1, Michael S Neuberger
• MGI ID: MGI:2447178
• Synonyms: mus^-, sIgM^-, Smu^-
• Gene: Ighm Location: Chr12:113382446-113386350 bp, - strand Genetic Position: Chr12, 62.1 cM, cytoband F1-2
• Alliance: Ighm^tm1Msn page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:49405
• Parent Cell Line: CCB (ES Cell)
• Strain of Origin: 129S/SvEv-Gpi1^c

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A secretory isoform specific mutation was achieved by deleting a 1.8 kb fragment encompassing sequence encoding a portion of the secretory tailpiece and the stop codon and polyadenylation sites. A single loxP site was left in place of the deleted sequence after the in vitro removal of the floxed tk-neo selection. Serum Igh-6 was undetectable in homozygous mutant mice. Flow cytometric analysis showed increased expression of the surface isoform. Study of heterozygous mice using allotype specific antibodies indicated that the augmented surface expression was a result of affected RNA processing rather than compensation. (J:49405)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ighm Mutation: 56 strains or lines available

References

• Original: J:49405 Ehrenstein MR, et al., Targeted gene disruption reveals a role for natural secretory IgM in the maturation of the primary immune response. Proc Natl Acad Sci U S A. 1998 Aug 18;95(17):10089-93
• All: 16 reference(s)