Igh^tm1Cog
Targeted Allele Detail

Summary

• Symbol: Igh^tm1Cog
• Name: immunoglobulin heavy chain complex; targeted mutation 1, Michel Cogne
• MGI ID: MGI:2447194
• Synonyms: JH-neo-Emu
• Gene: Igh Location: Chr12:113222388-115973574 bp, - strand Genetic Position: Chr12, Syntenic

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:80654
• Parent Cell Line: CK35 (ES Cell)
• Strain of Origin: 129S2/SvPas

Mutation description

• Allele Type: Targeted (Modified regulatory region, Null/knockout)
• Mutation: Insertion
• Mutation details: A loxP site flanked neomycin resistance gene cassette was inserted upstream of the E mu enhancer (Rr150) in the intergenic region following the Igh-J region, such that E mu and its flanking matrix-associated regions (MARs) were left intact. Flow cytometric analysis showed a lack of surface IgM⁺ B cells, while ELISA indicated an absence of all serum immunoglobulin classes. The insertion blocked transcription of the Igh-J region and prevented rearranged VDJ segments. Excision of the neo cassette resulted in rescue, indicating the observed phenotypes were the result of the presence of neo cassette rather than a deletion of a regulatory element. (J:80654)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Igh Mutation: 44 strains or lines available

References

• Original: J:80654 Delpy L, et al., B Cell Development Arrest Upon Insertion of a neo Gene Between J(H) and E micro : Promoter Competition Results in Transcriptional Silencing of Germline J(H) and Complete V(D)J Rearrangements. J Immunol. 2002 Dec 15;169(12):6875-82
• All: 2 reference(s)