Arhgap6^tm1Hzo
Targeted Allele Detail

Summary

• Symbol: Arhgap6^tm1Hzo
• Name: Rho GTPase activating protein 6; targeted mutation 1, Huda Y Zoghbi
• MGI ID: MGI:2447302
• Gene: Arhgap6 Location: ChrX:167578095-168087431 bp, + strand Genetic Position: ChrX, 78.77 cM
• Alliance: Arhgap6^tm1Hzo page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:80946
• Parent Cell Line: AB2.1 (ES Cell)
• Strain of Origin: 129S7/SvEvBrd-Hprt1^b-m2

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exons 6, 7, and 8 were replaced with a cassette containing a neomycin resistance gene, a single loxP site, and a 5' portion of an hprt minigene. Sequence analysis of RT-PCR products verified the deletion of the targeted exons and the splicing of exon 5 to exon 9, resulting in frameshift and consequent nonsense mutation. The transcript encodes a truncated protein containing 2 putative SH3-binding domains. The single loxP site was inserted for use as a centromeric anchor point for a larger deletion extending from the rhoGAP domain of Arhgap6 through a 5' untranslated exon of Mid1, recapitulating a deletion associated with human MLS. (J:80946)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Arhgap6 Mutation: 9 strains or lines available

References

• Original: J:80946 Prakash SK, et al., Functional analysis of ARHGAP6, a novel GTPase-activating protein for RhoA. Hum Mol Genet. 2000 Mar 1;9(4):477-88
• All: 2 reference(s)