Dmtn^tm1Ahc
Targeted Allele Detail

Summary

• Symbol: Dmtn^tm1Ahc
• Name: dematin actin binding protein; targeted mutation 1, Athar H Chishti
• MGI ID: MGI:2447353
• Synonyms: HPKO
• Gene: Dmtn Location: Chr14:70839624-70873488 bp, - strand Genetic Position: Chr14, 36.32 cM
• Alliance: Dmtn^tm1Ahc page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:76575
• Parent Cell Line: AK7 (ES Cell)
• Strain of Origin: 129S4/SvJaeSor

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exons 11 through 15 were replaced with a neomycin selection cassette inserted by homologous recombination. The deleted sequence encoded the carboxy terminal headpiece domain of the endogenous protein. RT-PCR analysis of kidney and brain tissues indicated an absence of normal transcript in homozygous mutant mice. A single truncated peptide species, corresponding to the targeted deletion, was identified in homozygous mutant mice by Western blot analysis. (J:76575)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Dmtn Mutation: 160 strains or lines available

References

• Original: J:76575 Khanna R, et al., Headpiece domain of dematin is required for the stability of the erythrocyte membrane. Proc Natl Acad Sci U S A. 2002 May 14;99(10):6637-42
• All: 4 reference(s)