Tg(hAGT)2041Sig
Transgene Detail

Summary

• Symbol: Tg(hAGT)2041Sig
• Name: transgene insertion 2041, Curt Sigmund
• MGI ID: MGI:2447399
• Synonyms: 204/1 line, A+, hAng, Hu-AGT-TG
• Transgene: Tg(hAGT)2041Sig Location: unknown
• Alliance: Tg(hAGT)2041Sig page

Transgene origin

• Strain of Origin: (C57BL/6 x SJL/J)F2

Transgene description

• Transgene Type: Transgenic (Humanized sequence, Inserted expressed sequence)
• Mutation: Insertion
• Tg(hAGT)2041Sig expresses 1 gene
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  AGT (183)

  Agt	(MGI:87963)

• Mutation details: The 14 kb transgenic construct contained the entire human angiotensinogen gene (approximately 11.5 kb) and 5' (1.2 kb) and 3' (1.4 kb) flanking sequence. Sequence analysis showed that the construct contained the M235T variant but not the T174M variant. The human AGT promoter drove high levels of expression in the liver and kidney. Expression was also detected in the heart, adrenal gland, and adipose tissue, and at very low levels in the brain and submandibular gland. Sexually dimorphic expression, in which expression was higher in males, was observed in the kidney and adrenal gland. Lines 204.1 (2041) and 204.3 (2043) were created and transgenic mice on a C57BL/6 background show angiotensinogen plasma concentrations 150X that normally found in human plasma. (J:79944)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:79944 Yang G, et al., Functional expression of the human angiotensinogen gene in transgenic mice. J Biol Chem. 1994 Dec 23;269(51):32497-502
• All: 37 reference(s)