Apoe^tm1Khw
Targeted Allele Detail

Summary

• Symbol: Apoe^tm1Khw
• Name: apolipoprotein E; targeted mutation 1, Karl H Weisgraber
• MGI ID: MGI:2447429
• Synonyms: Apoe^h, ApoeR61^h, ApoeR^h, Arg-61^neo+
• Gene: Apoe Location: Chr7:19430034-19433113 bp, - strand Genetic Position: Chr7, 9.94 cM
• Alliance: Apoe^tm1Khw page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:75681
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129S4/SvJae

Mutation description

• Allele Type: Targeted (Humanized sequence, Hypomorph)
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: A threonine to arginine substitution was introduced into amino acid 79 (p.T79R) of mouse Apoe exon 3 and a floxed neo cassette was inserted into the downstream intron via homologous recombination. This mutation allows the mouse protein to mimic human APOE4 domain interaction. The presence of the neo cassette renders this allele hypomorphic with mRNA expression at 2%-5% that of wild-type. Expression of the mutant allele was observed by Western blot analysis. (J:75681)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Apoe Mutation: 158 strains or lines available

References

• Original: J:75681 Raffai RL, et al., Hypomorphic Apolipoprotein E Mice. A NEW MODEL OF CONDITIONAL GENE REPAIR TO EXAMINE APOLIPOPROTEIN E-MEDIATED METABOLISM. J Biol Chem. 2002 Mar 29;277(13):11064-8
• All: 17 reference(s)