Slc9a2^tm1Ges
Targeted Allele Detail

Summary

• Symbol: Slc9a2^tm1Ges
• Name: solute carrier family 9 (sodium/hydrogen exchanger), member 2; targeted mutation 1, Gary E Shull
• MGI ID: MGI:2447441
• Synonyms: NHE2^-
• Gene: Slc9a2 Location: Chr1:40720872-40808045 bp, + strand Genetic Position: Chr1, 19.66 cM
• Alliance: Slc9a2^tm1Ges page

Structural abnormalities in the oxyntic mucosa of the gastric corpus in Slc9a2^tm1Ges/Slc9a2^tm1Ges mice

Show the 3 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:46545
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: The gene was disrupted by insertion of a neomycin resistance gene into exon 2, which encodes transmembrane domains 2-7 (amino acids 99-252). Northern blot analysis detected reduced levels of mutant transcript in kidney, small and large intestines, and high levels of mutant transcript in stomach of homozygous mutant animals. Sequence analysis revealed a cryptic splice donor site at codon 172 of exon 2 spliced to the acceptor of site exon 3. This alternative transcript results in a frameshift eliminating 614 out of 813 amino acids. The deleted region includes 9 out of 12 putative transmembrane domains and the carboxy terminal hydrophilic domain. (J:46545)

Expression

In Mice Carrying this Mutation:	1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Slc9a2 Mutation: 39 strains or lines available

References

• Original: J:46545 Schultheis PJ, et al., Targeted disruption of the murine Na+/H+ exchanger isoform 2 gene causes reduced viability of gastric parietal cells and loss of net acid secretion. J Clin Invest. 1998 Mar 15;101(6):1243-53
• All: 27 reference(s)