Tg(Amh-Smad4)1Tkw
Transgene Detail

Summary

• Symbol: Tg(Amh-Smad4)1Tkw
• Name: transgene insertion 1, Teresa K Woodruff
• MGI ID: MGI:2447446
• Synonyms: Tg(Amh-Madh4)1Tkw
• Transgene: Tg(Amh-Smad4)1Tkw Location: unknown
• Alliance: Tg(Amh-Smad4)1Tkw page

Transgene origin

• Strain of Origin: CD-1

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(Amh-Smad4)1Tkw expresses 1 gene
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  mouse	Smad4 (MGI:894293)

• Mutation details: The transgene consists of a Smad4 cDNA under the control of the anti-Mullerian hormone (Amh) promoter. A FLAG epitope tag and human growth hormone polyadenylation sequence was also added to the construct. Transgene expression was confirmed by Western blot analysis of testes extracts from neonatal transgenic mice. (J:79698)

Expression

In Structures Affected by this Mutation:

5 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

Notes

Transgenic line B showed the highest level of transgene expression and was used for further study.

References

• Original: J:79698 Narula A, et al., Smad4 overexpression causes germ cell ablation and leydig cell hyperplasia in transgenic mice. Am J Pathol. 2002 Nov;161(5):1723-34
• All: 1 reference(s)