Cd79a^tm2Cgn
Targeted Allele Detail

Summary

• Symbol: Cd79a^tm2Cgn
• Name: CD79A antigen (immunoglobulin-associated alpha); targeted mutation 2, University of Cologne
• MGI ID: MGI:2447454
• Synonyms: IgAlpha DeltaC, mb-1^DeltaC
• Gene: Cd79a Location: Chr7:24596922-24601283 bp, + strand Genetic Position: Chr7, 13.49 cM
• Alliance: Cd79a^tm2Cgn page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:80885
• Parent Cell Line: BL/6-III (ES Cell)
• Strain of Origin: C57BL/6

Mutation description

• Allele Type: Targeted
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: The translation of the immunoreceptor tyrosine-based activation motif (ITAM) was precluded by the generation of a nonsense mutation at codon 181 (exon 4). Sequence analysis revealed that the incorporation of a targeting vector containing a floxed neo cassette downstream of the endogenous locus and tyrosine to phenylalanine missense mutations in the ITAM resulted in a 5 nt deletion (4506 to 4510) thereby generating the stop codon at position 181. The neo cassette was excised in vitro via cre mediated recombination. The resultant protein was expected to lack 40 carboxy terminal amino acids, and thus retain B cell surface Ig expression but lack endogenous signaling. (J:80885)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cd79a Mutation: 24 strains or lines available

References

• Original: J:80885 Torres RM, et al., Aberrant B cell development and immune response in mice with a compromised BCR complex. Science. 1996 Jun 21;272(5269):1804-8
• All: 8 reference(s)