Tg(Igh-Pim1)1Brn
Transgene Detail

Summary

• Symbol: Tg(Igh-Pim1)1Brn
• Name: transgene insertion 1, Anton Berns
• MGI ID: MGI:2447531
• Synonyms: Emu-pim-1, Emu-Pim1
• Transgene: Tg(Igh-Pim1)1Brn Location: unknown
• Alliance: Tg(Igh-Pim1)1Brn page

Transgene origin

• Strain of Origin: (CBA x C57BL/6LiA)F2

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(Igh-Pim1)1Brn expresses 1 gene
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  mouse	Pim1 (MGI:97584)

• Mutation details: The transgene consists of a duplicated Igh enhancer (Emu), the Pim1 gene, and a mouse Moloney leukemia virus LTR. Transgene expression was confirmed by Northern and Western blot analysis. High expression of Pim1 mRNA was observed in the thymus, bone marrow, and spleen, but not in testis. Pim1 protein expression is observed in both B and T lymphocytes. (J:79624)

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

Notes

Transgenic lines ppG66 and ppG64 carrying 2-4 copies of the transgene were created. Both lines exhibit similar transgene expression patterns. The ppG64 line showed some transgene instability. Subline ppG64a resembles the original founder whereas subline ppG64b lacks one founder-specific band on the Southern blot.

References

• Original: J:79624 van Lohuizen M, et al., Predisposition to lymphomagenesis in pim-1 transgenic mice: cooperation with c-myc and N-myc in murine leukemia virus-induced tumors. Cell. 1989 Feb 24;56(4):673-82
• All: 17 reference(s)