Tg(SFTPC-RAF1-BxB)1Urr
Transgene Detail

Summary

• Symbol: Tg(SFTPC-RAF1-BxB)1Urr
• Name: transgene insertion 1, Ulf R Rapp
• MGI ID: MGI:2447567
• Synonyms: SP-C-c-raf-1-BxB, SP-C-craf-BxB, SP-C-craf BxB-23
• Transgene: Tg(SFTPC-RAF1-BxB)1Urr Location: unknown
• Alliance: Tg(SFTPC-RAF1-BxB)1Urr page

Transgene origin

• Strain of Origin: (C57BL/6 x DBA/2)F1

Transgene description

• Transgene Type: Transgenic (Constitutively active, Humanized sequence, Inserted expressed sequence)
• Mutation: Insertion
• Mutation details: The transgene is composed of a human SFTPC promoter, a human mutant RAF1 cDNA, and an SV40 small T antigen intron and polyadenylation signal. The mutant RAF1 sequence is deleted for the N-terminus and is constitutively active and highly transforming. 3.7 kb of human SFTPC promoter sequences was used to drive transgene expression in lung epithelium. The transgene is highly expressed in lungs as observed by Western blot analysis. The transgene is also weakly expressed in kidneys. (J:80895)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Tumor Data

List all tumor models in MMHCdb carrying Tg(SFTPC-RAF1-BxB)1Urr

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

Notes

2 independent transgenic lines were created.

References

• Original: J:80895 Kerkhoff E, et al., Lung-targeted expression of the c-Raf-1 kinase in transgenic mice exposes a novel oncogenic character of the wild-type protein. Cell Growth Differ. 2000 Apr;11(4):185-90
• All: 16 reference(s)