Tg(H2-K-BCL2)1Josd
Transgene Detail

Summary

• Symbol: Tg(H2-K-BCL2)1Josd
• Name: transgene insertion 1, Jos Domen
• MGI ID: MGI:2447580
• Synonyms: Bcl-2 Tg, H2K-BCL-2, H2K-BCL-2Tg
• Transgene: Tg(H2-K-BCL2)1Josd Location: unknown
• Alliance: Tg(H2-K-BCL2)1Josd page

Transgene origin

• Strain of Origin: (C57BL/6 x C3H)F1

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(H2-K-BCL2)1Josd expresses 1 gene
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  BCL2 (596)

  Bcl2	(MGI:88138)

• Mutation details: A human BCL2 cDNA under regulatory control of the H2-K promoter was used for the transgene. The H2-K promoter was used to drive transgene expression in hematopoietic stem cells. Northern blot analysis detected strong transgene expression in kidney, spleen, thymus, and lung. Weak expression was observed in the liver. Furthermore, Western blot analysis showed transgene expression in lymph nodes, heart, spleen, thymus, liver, and lungs. (J:80894)

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

Notes

Ten transgenic founders were created. Transgenic founders 1038, 1043, and 1053 were used for analysis. All founders tested exhibit similar patterns of transgene expression.

References

• Original: J:80894 Domen J, et al., Systemic overexpression of BCL-2 in the hematopoietic system protects transgenic mice from the consequences of lethal irradiation. Blood. 1998 Apr 1;91(7):2272-82
• All: 43 reference(s)