Tg(LCKprBCL2L1)12Sjk
Transgene Detail

Summary

• Symbol: Tg(LCKprBCL2L1)12Sjk
• Name: transgene insertion 12, Stanley J Korsmeyer
• MGI ID: MGI:2447593
• Synonyms: Bcl-xL, Bcl-xL-Tg, Bcl-xL^Tg, lck^pr-bcl-xL
• Transgene: Tg(LCKprBCL2L1)12Sjk Location: unknown
• Alliance: Tg(LCKprBCL2L1)12Sjk page

Transgene origin

• Strain of Origin: Not Specified

Transgene description

• Transgene Type: Transgenic (Humanized sequence, Inserted expressed sequence)
• Mutation: Insertion
• Tg(LCKprBCL2L1)12Sjk expresses 1 gene
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  BCL2L1 (598)

  Bcl2l1

  (MGI:88139)

• Mutation details: The transgene consists of 0.75 kb of Lck proximal promoter sequences and 0.8 kb of human BCL2L1 cDNA. Introns, exons, and polyadenylation sequences from the human growth hormone gene consitutes the 3' untranslated region of the construct. The Lck promoter was used to drive transgene expression in lymphoid tissues. Transgene expression in the thymus and spleen was verified by Western blot analysis and flow cytometry. (J:78583)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

Notes

Transgenic lines 12 and 24, which expressed high amounts of the transgene, were established. Most of the analysis was performed using line 12 and confirmed with line 24.

References

• Original: J:78583 Chao DT, et al., Bcl-XL and Bcl-2 repress a common pathway of cell death. J Exp Med. 1995 Sep 1;182(3):821-8
• All: 36 reference(s)