Pold1^tm1Bdp
Targeted Allele Detail

Summary

• Symbol: Pold1^tm1Bdp
• Name: polymerase (DNA directed), delta 1, catalytic subunit; targeted mutation 1, Bradley D Preston
• MGI ID: MGI:2447628
• Synonyms: exo-, Pold1^D400A, Pold1^tm2Bdp
• Gene: Pold1 Location: Chr7:44182168-44198239 bp, - strand Genetic Position: Chr7, 28.83 cM
• Alliance: Pold1^tm1Bdp page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:100685
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted
• Mutation: Nucleotide substitutions
• Mutation details: A point mutation resulting in an aspartic acid to alanine substition at residue 400 (D400A) was introduced at the endogenous locus by homologous recombination of a targeting vector also carrying an intronic neo cassette. The missense mutation was introduced into sequence encoding the proofreading domain. RT-PCR analysis of heterozygous ES cells showed comparable levels of wild-type and mutant transcript. Western blot analysis of fibroblasts derived from mutant mice further indicated normal levels of expression. (J:80306)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Tumor Data

List all tumor models in MMHCdb carrying Pold1^tm1Bdp

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Pold1 Mutation: 63 strains or lines available

References

• Original: J:100685 Goldsby RE, et al., Defective DNA polymerase-delta proofreading causes cancer susceptibility in mice. Nat Med. 2001 Jun;7(6):638-9
• All: 3 reference(s)