Del(17Abcg5-Abcg8)1Hobb
Targeted Allele Detail

Summary

• Symbol: Del(17Abcg5-Abcg8)1Hobb
• Name: deletion, Chr 17, Helen H Hobbs 1; targeted mutation 1, Helen H Hobbs
• MGI ID: MGI:2447644
• Synonyms: Abcg5/Abcg8^tm1Hobb, G5G8^-
• Gene: Del(17Abcg5-Abcg8)1Hobb Location: unknown Genetic Position: Chr17, Syntenic

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:80679
• Parent Cell Line: SM1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intergenic deletion, Intragenic deletion
• Del(17Abcg5-Abcg8)1Hobb involves 2 genes/genome features (Abcg5, Abcg8) View all
• Mutation details: Both the Abcg5 and Abcg8 endogenous loci, which are oriented head to head and separated by approximately 400 bp, were disrupted by the integration of a single targeting construct. A neomycin selection cassette flanked by both loxP and frt sites replaced sequence extending from Abcg5 intron 2 into Abcg8 intron 3. The Walker A consensus sequences that make up a portion of the ATP binding sites in both genes were deleted. Northern and Western blot analyses indicated an absence of normal transcript and protein derived from both loci in homozygous mutant mice. (J:80679)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Del(17Abcg5-Abcg8)1Hobb Mutation: 1 strain or line available

References

• Original: J:80679 Yu L, et al., Disruption of Abcg5 and Abcg8 in mice reveals their crucial role in biliary cholesterol secretion. Proc Natl Acad Sci U S A. 2002 Dec 10;99(25):16237-42
• All: 19 reference(s)