Tg(NOS3)2Crom
Transgene Detail

Summary

• Symbol: Tg(NOS3)2Crom
• Name: transgene insertion 2, Rini de Crom
• MGI ID: MGI:2447657
• Synonyms: eNOStg2
• Transgene: Tg(NOS3)2Crom Location: unknown
• Alliance: Tg(NOS3)2Crom page

Transgene origin

• Strain of Origin: FVB

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(NOS3)2Crom expresses 1 gene
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  NOS3 (4846)

  Nos3	(MGI:97362)

• Mutation details: The entire human NOS3 genomic sequence and flanking regions was used for the transgene. Transgene expression was confirmed by Western blot analysis of aorta homogenates. Transgene expression was also observed by immunohistochemistry. Blood vessels and capillaries of heart sections from transgenic animals stained positive for human NOS3, as did sinusoids of the liver and kidney and capillaries of the glomeruli. (J:80701)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

Notes

Two transgenic lines, eNOStg2 and eNOStg3, were created. Line eNOStg2 expresses the highest amount of human transgene product. In the aorta, eNOStg2 expresses 10-fold the amount of active enzyme compared to wild-type.

References

• Original: J:80701 van Haperen R, et al., Reduction of blood pressure, plasma cholesterol, and atherosclerosis by elevated endothelial nitric oxide. J Biol Chem. 2002 Dec 13;277(50):48803-7
• All: 4 reference(s)