Tg(BLG-STAT5A)15Itb
Transgene Detail

Summary

• Symbol: Tg(BLG-STAT5A)15Itb
• Name: transgene insertion 15, Itamar Barash
• MGI ID: MGI:2447712
• Synonyms: BGL/STAT5ca, Tg(BLG-STAT5A)2Itb
• Transgene: Tg(BLG-STAT5A)15Itb Location: unknown
• Alliance: Tg(BLG-STAT5A)15Itb page

Transgene origin

• Strain of Origin: FVB/N

Transgene description

• Transgene Type: Transgenic (Constitutively active, Inserted expressed sequence)
• Mutation: Insertion
• Mutation details: The transgene consists of a beta-lactoglobulin promoter, active in mammary glands, and a fusion gene comprised of amino acids 1-750 of the sheep STAT5A gene, the terminal transactivation domain (amino acids 677-847) of human STAT6, and the kinase domain (amino acids 757-1129) of mouse Jak2. The resulting protein is a constitutively active STAT5a molecule with self-activating properties. Transgene expression was confirmed by Western blot and RT-PCR analysis of mammary glands from transgenic animals at day 6 of lactation. (J:80588)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

Notes

This is transgenic line BGL/STAT5ca-15. In addition to line 15, J:80588 shows data for lines BGL/STAT5ca-8, and BGL/STAT5ca-19. Three other transgenic lines were created (BGL/STAT5ca-7, -10, and -15). Lines BGL/STAT5ca-8 and -15 carry 10 copies of the integrated transgene, line BGL/STAT5ca-7 carries 5 copies, line BGL/STAT5ca-10 carries 6 copies, and line BGL/STAT5ca-19 carries 3 copies.

References

• Original: J:80588 Iavnilovitch E, et al., Overexpression and forced activation of stat5 in mammary gland of transgenic mice promotes cellular proliferation, enhances differentiation, and delays postlactational apoptosis. Mol Cancer Res. 2002 Nov;1(1):32-47
• All: 3 reference(s)