Lta/Tnf^tm1Fda
Targeted Allele Detail

Summary

• Symbol: Lta/Tnf^tm1Fda
• Name: lymphotoxin A; targeted mutation 1, Francois Dautry
• MGI ID: MGI:2447728
• Synonyms: TNF^-LT^-
• Gene: Lta Location: Chr17:35422141-35424327 bp, - strand Genetic Position: Chr17, 18.59 cM
• Alliance: Lta/Tnf^tm1Fda page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:39519
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intergenic deletion
• Mutation details: The entire Lta gene and the majority of the Tnf gene (to codon 113 in exon 4) were replaced by a neomycin selection cassette inserted by homologous recombination. No transcript or truncated protein was detected by a variety of assays including Northern blot analysis of concanavalin A stimulated splenocytes and ELISA of macrophage colony supernatants. Tnf activity was undetected in LPS stimulated homozygous mutant mice. (J:39519)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Lta Mutation: 17 strains or lines available

References

• Original: J:39519 Amiot F, et al., Mice heterozygous for a deletion of the tumor necrosis factor-alpha and lymphotoxin-alpha genes: biological importance of a nonlinear response of tumor necrosis factor-alpha to gene dosage. Eur J Immunol. 1997 Apr;27(4):1035-42
• All: 7 reference(s)