Ikzf1^{tm1.1(Pax5)Mbu}
Targeted Allele Detail

Summary

• Symbol: Ikzf1^{tm1.1(Pax5)Mbu}
• Name: IKAROS family zinc finger 1; targeted mutation 1.1, Meinrad Busslinger
• MGI ID: MGI:2447787
• Synonyms: Ik^Pax5
• Gene: Ikzf1 Location: Chr11:11634980-11722926 bp, + strand Genetic Position: Chr11, 7.02 cM
• Alliance: Ikzf1^{tm1.1(Pax5)Mbu} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:80815
• Parent Cell Line: HM-1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd-Hprt1^b-m3

Mutation description

• Allele Type: Targeted (Inserted expressed sequence, Null/knockout)
• Mutation: Insertion
• Mutation details: The floxed neo cassette in exon 2 was removed by mating Znfn1a1^tm1(Pax)Mbu animals to cre expressing line TgN(Sycp1-cre)4Min, thus allowing expression from the Pax5-GFP cassette inserted at Znfn1a1. Western blot analysis detected Pax5 protein expression in thymocytes and myeloid cells at levels similar to wild-type. GFP expression in B cells was detected by flow cytometric analysis. (J:80815)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ikzf1 Mutation: 30 strains or lines available

References

• Original: J:80815 Souabni A, et al., Pax5 promotes B lymphopoiesis and blocks T cell development by repressing Notch1. Immunity. 2002 Dec;17(6):781-93
• All: 5 reference(s)