Cit^tm1Fdc
Targeted Allele Detail

Summary

• Symbol: Cit^tm1Fdc
• Name: citron; targeted mutation 1, Ferdinando Di Cunto
• MGI ID: MGI:2447844
• Synonyms: CIT-K^-, Citron-K^-
• Gene: Cit Location: Chr5:115983337-116147006 bp, + strand Genetic Position: Chr5, 56.1 cM, cytoband F
• Alliance: Cit^tm1Fdc page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:77297
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exon 2 of the endogenous locus was replaced with a cassette containing Beta-gal and neo genes, resulting in an isoform-specific mutation. Exon 2 is the second coding exon of the Citron-K isoform and is far upstream of the Citron-N trascriptional start site. Western blot analysis of cerebral, splenic, and thymic tissues inidicated an absence of the Citron-K isoform and showed normal levels of the Citron-N isoform in homozygous mutant mice. (J:77297)

Expression

In Mice Carrying this Mutation:	6 assay results 1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	11 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cit Mutation: 121 strains or lines available

References

• Original: J:77297 Di Cunto F, et al., Defective neurogenesis in citron kinase knockout mice by altered cytokinesis and massive apoptosis. Neuron. 2000 Oct;28(1):115-27
• All: 8 reference(s)