Gclm^tm1Tdal
Targeted Allele Detail

Summary

• Symbol: Gclm^tm1Tdal
• Name: glutamate-cysteine ligase, modifier subunit; targeted mutation 1, Timothy P Dalton
• MGI ID: MGI:2447852
• Synonyms: Gclm(-), GCLM^-
• Gene: Gclm Location: Chr3:122039206-122060864 bp, + strand Genetic Position: Chr3, 52.94 cM, cytoband H1-3
• Alliance: Gclm^tm1Tdal page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:80927
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exon 1 was disrupted by the insertion of a neomycin selection cassette. The 3' splice donor site of intron 1 was deleted and translation from either of the putative start codons is expected to terminate after 61 residues. Neither normal transcript nor normal protein was detected by Northern and Western blot analyses of liver and kidney tissue obtained from homozygous mutant mice. (J:80927)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gclm Mutation: 16 strains or lines available

Notes

ES cell line = J1 (129S4/SvJae) or D, which is E14TG2a (129P2/OlaHsd).

References

• Original: J:80927 Yang Y, et al., Initial Characterization of the Glutamate-Cysteine Ligase Modifier Subunit Gclm(-/-) Knockout Mouse. NOVEL MODEL SYSTEM FOR A SEVERELY COMPROMISED OXIDATIVE STRESS RESPONSE. J Biol Chem. 2002 Dec 20;277(51):49446-52
• All: 27 reference(s)