Tg(Pcp2-ATXN1*30Q)2Horr
Transgene Detail

Summary

• Symbol: Tg(Pcp2-ATXN1*30Q)2Horr
• Name: transgene insertion 2, Harry T Orr
• MGI ID: MGI:2447853
• Synonyms: A02, ATXN1[30Q]-S776, PS-30, SCA1[30Q], Tg(Pcp2-SCA1)PS30Horr
• Transgene: Tg(Pcp2-ATXN1*30Q)2Horr Location: unknown
• Alliance: Tg(Pcp2-ATXN1*30Q)2Horr page

Transgene origin

• Strain of Origin: FVB/N

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(Pcp2-ATXN1*30Q)2Horr expresses 1 gene
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  ATXN1 (6310)

  Atxn1

  (MGI:104783)

• Mutation details: The transgene consists of a mouse Pcp2 promoter and a human wild-type SCA1 cDNA sequence with 30 CAG repeats. The 835 bp fragment of mouse Pcp2 regulatory sequence include exon 1, intron 1, and part of exon 2 of Pcp2. The Pcp2 promoter targets transgene expression to Purkinje cells. Transgene expression was confirmed by Northern blot and Western blot analysis of cerebellar extracts. (J:28932)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

Notes

Seven transgenic lines were created carrying between 5-200 copies of the integrated transgene. Lines A02, A06.1, and A06.2 exhibited the highest levels of transgene mRNA expression at 50- to 100-fold compared to the endogenous gene.

References

• Original: J:28932 Burright EN, et al., SCA1 transgenic mice: a model for neurodegeneration caused by an expanded CAG trinucleotide repeat. Cell. 1995 Sep 22;82(6):937-48
• All: 16 reference(s)