Igf2r^tm1.1Rlj
Targeted Allele Detail

Summary

• Symbol: Igf2r^tm1.1Rlj
• Name: insulin-like growth factor 2 receptor; targeted mutation 1.1, Randy L Jirtle
• MGI ID: MGI:2447858
• Gene: Igf2r Location: Chr17:12901293-12988551 bp, - strand Genetic Position: Chr17, 8.64 cM, cytoband A-C
• Alliance: Igf2r^tm1.1Rlj page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:80932
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129/Sv

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The floxed fragment containing exon 10 was excised from Igf2r^tm1Rlj via in vivo cre mediated recombination. Sequence analysis indicated that the deletion of exon 10 will cause a shift in the open reading frame resulting in a stop codon upstream of the mannose 6-phosphate binding site, the Igf2 protein product binidng site, and the transmembrane domain. While, protein was undetected in mutant embryos by Western blot analysis, immunohistochemical analysis showed protein expression in developing bronchiolar structures. This staining putatively represents expression from an unsilenced paternal allele. (J:80932)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Igf2r Mutation: 99 strains or lines available

References

• Original: J:80932 Wylie AA, et al., Tissue-specific inactivation of murine M6P/IGF2R. Am J Pathol. 2003 Jan;162(1):321-8
• All: 1 reference(s)