Car9^tm1Spas
Targeted Allele Detail

Summary

• Symbol: Car9^tm1Spas
• Name: carbonic anhydrase 9; targeted mutation 1, Silvia Pastorekova
• MGI ID: MGI:2448129
• Synonyms: CA IX KO, Car9^-
• Gene: Car9 Location: Chr4:43507026-43513729 bp, + strand Genetic Position: Chr4, 23.05 cM
• Alliance: Car9^tm1Spas page

Morphological changes in the stomach and intestine of Car2ⁿ/Car2ⁿ and Car9^tm1Spas/Car9^tm1Spas mice

Show the 1 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:80329
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A PGK-neo cassette was inserted into exon 1 by homologous recombination. The insertion of neo downstream of the signal peptide deleted 14 nt (268-282) of the proteoglycan domain and caused a frameshift mutation. RT-PCR analysis of homozygous mutant mice identified a frame shifted transcript lacking 5' sequence encoding the signal peptide and most of the proteoglycan domain. Though the mutant transcript contained 77 nt of the neo cassette, a 3' portion of exon 1, and all of exons 2 through 11, protein was undetected by Western blot analysis of stomach tissue. (J:80329)

Expression

In Mice Carrying this Mutation:	2 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	5 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Car9 Mutation: 46 strains or lines available

References

• Original: J:80329 Gut MO, et al., Gastric hyperplasia in mice with targeted disruption of the carbonic anhydrase gene Car9. Gastroenterology. 2002 Dec;123(6):1889-903
• All: 10 reference(s)