Nr6a1^tm2Coo
Targeted Allele Detail

Summary

• Symbol: Nr6a1^tm2Coo
• Name: nuclear receptor subfamily 6, group A, member 1; targeted mutation 2, Austin J Cooney
• MGI ID: MGI:2448158
• Synonyms: GCNF^lox
• Gene: Nr6a1 Location: Chr2:38613381-38816464 bp, - strand Genetic Position: Chr2, 24.42 cM
• Alliance: Nr6a1^tm2Coo page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:80978
• Parent Cell Line: AB1 (ES Cell)
• Strain of Origin: 129S7/SvEvBrd-Hprt1⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: The 243 bp exon encoding the DNA-binding domain was replaced by a single loxP site after cre mediated recombination within ES cells. A truncated protein, lacking the DNA-binding domain but retaining the downstream ligand-binding domain was detected by Western blot analysis of homozygous mutant mice. (J:80978)

Expression

In Mice Carrying this Mutation:	8 assay results
In Structures Affected by this Mutation:	8 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Nr6a1 Mutation: 145 strains or lines available

References

• Original: J:80978 Lan ZJ, et al., The embryonic function of germ cell nuclear factor is dependent on the DNA binding domain. J Biol Chem. 2002 Dec 27;277(52):50660-7
• All: 1 reference(s)