Zyx^tm1Mbk
Targeted Allele Detail

Summary

• Symbol: Zyx^tm1Mbk
• Name: zyxin; targeted mutation 1, Mary C Beckerle
• MGI ID: MGI:2448217
• Synonyms: zyxin^-
• Gene: Zyx Location: Chr6:42326564-42337147 bp, + strand Genetic Position: Chr6, 20.64 cM
• Alliance: Zyx^tm1Mbk page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:81007
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129X1/SvJ

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The gene was disrupted by replacement of a 4.5 kb region of the gene containing a 5' non-coding exon and the first 4 exons encoding amino acids 1-333. Absence of gene expression was confirmed by Western blot analysis of lung and spleen mRNA from homozygous mutant animals. Multiple antibodies directed against N-terminal, central, and C-terminal residues did not detect any protein product. (J:81007)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Zyx Mutation: 21 strains or lines available

Notes

The authors report the 129SvJ GS embryonic stem cell line (Genome Systems, Inc., St.Louis, MO) was used for gene targeting.

References

• Original: J:81007 Hoffman LM, et al., Targeted disruption of the murine zyxin gene. Mol Cell Biol. 2003 Jan;23(1):70-9
• All: 10 reference(s)