Sirt1^tm1Mcby
Targeted Allele Detail

Summary

• Symbol: Sirt1^tm1Mcby
• Name: sirtuin 1; targeted mutation 1, Michael W McBurney
• MGI ID: MGI:2448246
• Synonyms: Sir2-, sir2alpha null, SIRT1^-, Sirt1 KO
• Gene: Sirt1 Location: Chr10:63154784-63174814 bp, - strand Genetic Position: Chr10, 32.57 cM
• Alliance: Sirt1^tm1Mcby page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:81010
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The gene was disrupted by replacement of exons 5 and 6, which encode a large portion of the catalytic domain, with a hygromycin resistance cassette via homologous recombination. Northern blot analysis of RNA from homozygous mutant E12.5 embryos and adult tissues did not detect gene expression. (J:81010)

Disease models

Expression

In Mice Carrying this Mutation:	7 assay results 3 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	21 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Sirt1 Mutation: 81 strains or lines available

References

• Original: J:81010 McBurney MW, et al., The mammalian SIR2alpha protein has a role in embryogenesis and gametogenesis. Mol Cell Biol. 2003 Jan;23(1):38-54
• All: 71 reference(s)