Prkaa2^tm1.1Vio
Targeted Allele Detail

Summary

• Symbol: Prkaa2^tm1.1Vio
• Name: protein kinase, AMP-activated, alpha 2 catalytic subunit; targeted mutation 1.1, Benoit Viollet
• MGI ID: MGI:2448467
• Synonyms: alpha2 AMPK KO, alpha2KO, AMPK^-, Ampka2^-, AMPKalpha2^-
• Gene: Prkaa2 Location: Chr4:104887071-104967087 bp, - strand Genetic Position: Chr4, 49.05 cM
• Alliance: Prkaa2^tm1.1Vio page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:81120
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129S2/SvPas

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The floxed region containing the exon encoding amino acids 189 through 260 and a downstream neo cassette was excised from Prkaa2^tm1Vio in the germline via in vivo cre-mediated recombination. Protein was undetected by Western blot analysis of liver and muscle tissue obtained from homozygous mutant mice. (J:81120)

Expression

In Mice Carrying this Mutation:	1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	5 anatomical structure(s)

Tumor Data

List all tumor models in MMHCdb carrying Prkaa2^tm1.1Vio

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Prkaa2 Mutation: 35 strains or lines available

Notes

ES cell line = MPI-I (MPI1).

References

• Original: J:81120 Viollet B, et al., The AMP-activated protein kinase alpha2 catalytic subunit controls whole-body insulin sensitivity. J Clin Invest. 2003 Jan;111(1):91-8
• All: 112 reference(s)