Fmn2^tm1Led
Targeted Allele Detail

Summary

• Symbol: Fmn2^tm1Led
• Name: formin 2; targeted mutation 1, Philip Leder
• MGI ID: MGI:2448909
• Synonyms: Fmn2^-
• Gene: Fmn2 Location: Chr1:174329391-174650295 bp, + strand Genetic Position: Chr1, 81.04 cM
• Alliance: Fmn2^tm1Led page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:81246
• Parent Cell Line: TC1/TC-1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: The gene was disrupted by replacement of 1300 bp of the proline-rich exon with a neomycin resistance cassette via homologous recombination, introducing stop codons in all 3 reading frames. The deleted region encodes 433 amino acids and contains the FH1 domain. Northern blot analysis of brain RNA from homozygous mutants confirmed the absence of wild-type gene product. A partial read-through transcript containing the neomycin cassette was observed. (J:81246)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Fmn2 Mutation: 76 strains or lines available

References

• Original: J:81246 Leader B, et al., Formin-2, polyploidy, hypofertility and positioning of the meiotic spindle in mouse oocytes. Nat Cell Biol. 2002 Dec;4(12):921-8
• All: 18 reference(s)