Nkx2-5^tm2(cre)Rph
Targeted Allele Detail

Summary

• Symbol: Nkx2-5^tm2(cre)Rph
• Name: NK2 homeobox 5; targeted mutation 2, Richard P Harvey
• MGI ID: MGI:2448972
• Synonyms: Nkx2.5-Cre, Nkx2-5^IRESCre
• Gene: Nkx2-5 Location: Chr17:27057638-27063962 bp, - strand Genetic Position: Chr17, 13.6 cM
• Alliance: Nkx2-5^tm2(cre)Rph page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:80725
• Parent Cell Line: W9.5/W95 (ES Cell)
• Strain of Origin: 129S1/Sv-Oca2⁺ Tyr⁺ Kitl⁺

Mutation description

• Allele Type: Targeted (No functional change, Recombinase)
• Mutation: Insertion
• Nkx2-5^tm2(cre)Rph expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Nkx2-5 (MGI:97350)

• Mutation details: Sequence encoding cre recombinase adjoined to an IRES was inserted into the 3' UTR of the endogenous locus via homologous recombination. A single frt site remained in the UTR after an frt-flanked hygromycin selection cassette was excised. A lack of observed allele complementation indicated that the inserted sequence did not significantly affect expression of the endogenous locus. (J:80725)

Generation of the Smyd2^tm1.1Fben allele

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Nkx2-5 (mouse) Summary of all recombinase alleles driven by Nkx2-5. Comparative matrix view of recombinase activities Alliance mouse Nkx2-5 gene page

Expression

In Mice Carrying this Mutation:	203 assay results 12 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Nkx2-5 Mutation: 21 strains or lines available

References

• Original: J:80725 Stanley EG, et al., Efficient Cre-mediated deletion in cardiac progenitor cells conferred by a 3'UTR-ires-Cre allele of the homeobox gene Nkx2-5. Int J Dev Biol. 2002;46(4):431-9
• All: 78 reference(s)