Spib^tm1Mcs
Targeted Allele Detail

Summary

• Symbol: Spib^tm1Mcs
• Name: Spi-B transcription factor (Spi-1/PU.1 related); targeted mutation 1, M Celeste Simon
• MGI ID: MGI:2448991
• Synonyms: Spi-B-
• Gene: Spib Location: Chr7:44175417-44181495 bp, - strand Genetic Position: Chr7, 28.83 cM
• Alliance: Spib^tm1Mcs page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:45245
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The gene was disrupted by replacement of the DNA-binding domain-containing exon 6 and part of intron 5 with a PGK-neo cassette via homologous recombination. Northern blot analysis of homozygous mutant splenocyte RNA (using a full-length Spib cDNA probe) confirmed the absence of gene expression. (J:45245)

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Spib Mutation: 23 strains or lines available

References

• Original: J:45245 Su GH, et al., Defective B cell receptor-mediated responses in mice lacking the Ets protein, Spi-B. EMBO J. 1997 Dec 1;16(23):7118-29
• All: 22 reference(s)