Cenpe^tm1Dwc
Targeted Allele Detail

Summary

• Symbol: Cenpe^tm1Dwc
• Name: centromere protein E; targeted mutation 1, Don W Cleveland
• MGI ID: MGI:2449007
• Synonyms: CENP-E null
• Gene: Cenpe Location: Chr3:134918324-134979301 bp, + strand Genetic Position: Chr3, 62.55 cM, cytoband H2
• Alliance: Cenpe^tm1Dwc page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:81046
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: A 120 bp exon of the gene was flanked with a loxP site and a floxed PGK-neo cassette. Transient expression of cre recombinase in correctly targeted ES cells resulted in deletion of this floxed exon and is predicted to create a premature stop codon at amino acid 82, thus producing a non-functional protein product. (J:81046)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cenpe Mutation: 109 strains or lines available

References

• Original: J:81046 Putkey FR, et al., Unstable kinetochore-microtubule capture and chromosomal instability following deletion of CENP-E. Dev Cell. 2002 Sep;3(3):351-65
• All: 3 reference(s)