Nfia^tm1Rmg
Targeted Allele Detail

Summary

• Symbol: Nfia^tm1Rmg
• Name: nuclear factor I/A; targeted mutation 1, Richard M Gronostajski
• MGI ID: MGI:2449024
• Synonyms: Nfia^-
• Gene: Nfia Location: Chr4:97660971-98007111 bp, + strand Genetic Position: Chr4, 45.52 cM, cytoband C4-C6
• Alliance: Nfia^tm1Rmg page

Hydrocephalus in Nfia^tm1Rmg/Nfia^tm1Rmg mice

Show the 8 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:58137
• Parent Cell Line: E14.1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The gene was disrupted by replacement of 219 bp of exon 2 and the 3' splice acceptor with a PGK-neo cassette via homologous recombination. The mutant allele generates a fusion between exons 1 and 3 resulting in an out-of-frame transcript with a nonsense codon in exon 3. The mutant allele was verified by RT-PCR and sequence analysis. (J:58137)

Expression

In Mice Carrying this Mutation:	3 assay results 3 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	11 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Nfia Mutation: 28 strains or lines available

References

• Original: J:58137 das Neves L, et al., Disruption of the murine nuclear factor I-A gene (Nfia) results in perinatal lethality, hydrocephalus, and agenesis of the corpus callosum. Proc Natl Acad Sci U S A. 1999 Oct 12;96(21):11946-51
• All: 25 reference(s)