Pck1^tm1Mgn
Targeted Allele Detail

Summary

• Symbol: Pck1^tm1Mgn
• Name: phosphoenolpyruvate carboxykinase 1, cytosolic; targeted mutation 1, Mark A Magnuson
• MGI ID: MGI:2449282
• Synonyms: pck^lox+neo
• Gene: Pck1 Location: Chr2:172994866-173001067 bp, + strand Genetic Position: Chr2, 95.79 cM, cytoband C1-qter
• Alliance: Pck1^tm1Mgn page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:63929
• Parent Cell Line: TL1/TL-1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Conditional ready, Hypomorph)
• Mutation: Insertion
• Mutation details: A loxP-flanked neomycin selection cassette was inserted into intron 3 and a single loxP site was inserted into intron 5. Activity and protein mass assays on protein derived from kidney and liver of fasted homozygous mice demonstrated that this allele behaves as a hypomorph, retaining about 10-20% of normal activity. (J:63929)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Pck1 Mutation: 47 strains or lines available

References

• Original: J:63929 She P, et al., Phosphoenolpyruvate carboxykinase is necessary for the integration of hepatic energy metabolism. Mol Cell Biol. 2000 Sep;20(17):6508-17
• All: 2 reference(s)