Hprt1^tm1(AGT)Sig
Targeted Allele Detail

Summary

• Symbol: Hprt1^tm1(AGT)Sig
• Name: hypoxanthine phosphoribosyltransferase 1; targeted mutation 1, Curt Sigmund
• MGI ID: MGI:2449451
• Synonyms: hAGT^AT
• Gene: Hprt1 Location: ChrX:52077014-52110536 bp, + strand Genetic Position: ChrX, 29.31 cM, cytoband A6
• Alliance: Hprt1^tm1(AGT)Sig page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:81310
• Parent Cell Line: BK4 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Humanized sequence, Inserted expressed sequence)
• Mutation: Insertion
• Hprt1^tm1(AGT)Sig expresses 1 gene
  Knock-in expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  AGT (183)

  Agt	(MGI:87963)

• Mutation details: The entire human AGT genomic sequence containing an A to G nucleotide substitution at position -6 of the promoter was inserted into the Hprt locus via homologous recombination. (This polymorphism has been reported in association with hypertension in humans.) RNase protection assay showed transgene expression in liver, kidneys, brain, and aorta. In addition, immunohistochemical analysis and Western blot detected transgene expression in the kidneys and plasma, respectively. (J:81310)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Hprt1 Mutation: 1279 strains or lines available

References

• Original: J:81310 Cvetkovic B, et al., Physiological significance of two common haplotypes of human angiotensinogen using gene targeting in the mouse. Physiol Genomics. 2002 Dec 3;11(3):253-62
• All: 2 reference(s)