Hprt1^tm2(AGT)Sig
Targeted Allele Detail

Summary

• Symbol: Hprt1^tm2(AGT)Sig
• Name: hypoxanthine phosphoribosyltransferase 1; targeted mutation 2, Curt Sigmund
• MGI ID: MGI:2449453
• Synonyms: hAGT^GM, hAGT^hprt
• Gene: Hprt1 Location: ChrX:52077014-52110536 bp, + strand Genetic Position: ChrX, 29.31 cM, cytoband A6
• Alliance: Hprt1^tm2(AGT)Sig page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:81515
• Parent Cell Line: BK4 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Humanized sequence, Inserted expressed sequence)
• Mutation: Insertion
• Hprt1^tm2(AGT)Sig expresses 1 gene
  Knock-in expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  AGT (183)

  Agt	(MGI:87963)

• Mutation details: The entire human AGT genomic sequence containing a C to T nucleotide substitution at codon 235 was inserted into the Hprt locus via homologous recombination. (This polymorphism results in a threonine to methionine substitution and has been reported in association with hypertension in humans.) Northern blot analysis showed high transgene expression in liver and kidneys, and lower levels of transgene expression in brain, white and brown adipose tissue, diaphram, and aorta. In addition, immunohistochemical analysis of kidney and liver sections detected transgene expression in the proximal tubule cells of the renal cortex and in hepatocytes. (J:81515)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Hprt1 Mutation: 1279 strains or lines available

References

• Original: J:81515 Cvetkovic B, et al., Appropriate tissue- and cell-specific expression of a single copy human angiotensinogen transgene specifically targeted upstream of the HPRT locus by homologous recombination. J Biol Chem. 2000 Jan 14;275(2):1073-8
• All: 3 reference(s)