Ntan1^tm1Avar
Targeted Allele Detail

Summary

• Symbol: Ntan1^tm1Avar
• Name: N-terminal Asn amidase; targeted mutation 1, Alexander Varshavsky
• MGI ID: MGI:2449464
• Synonyms: Ntan1^-
• Gene: Ntan1 Location: Chr16:13636709-13653315 bp, + strand Genetic Position: Chr16, 9.64 cM
• Alliance: Ntan1^tm1Avar page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:62140
• Parent Cell Line: CJ7 (ES Cell)
• Strain of Origin: 129S1/Sv-Oca2⁺ Tyr⁺ Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The gene was disrupted by replacement of exons 2-5 with a PGK-neo cassette via homologous recombination. Northern blot analysis detected no expression in brains of homozygous mutant animals, but testes mRNA contained transcripts which apparently lack the 5' half of the open reading frame. Whole mount in-situ hybridization analysis of E9.75 homozygous mutant embryos did not detect any gene expression. (J:62140)

Expression

In Mice Carrying this Mutation:

20 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ntan1 Mutation: 16 strains or lines available

References

• Original: J:62140 Kwon YT, et al., Altered activity, social behavior, and spatial memory in mice lacking the NTAN1p amidase and the asparagine branch of the N-end rule pathway. Mol Cell Biol. 2000 Jun;20(11):4135-48
• All: 5 reference(s)